The Chemistry Department NMR Facility is located in Chemistry B39, and is used by researchers to characterize small molecules. The facility contains a Bruker Avance 500 MHz NMR spectrometer equipped with BBO and BBI probes. Data can be collected over a temperature range of -80 °C to 100 °C. Users must have an account to access the instrument. Members of the Chemistry department are charged at a rate of $9/h and users from outside the department at $30/h. New users must complete an initial NMR training session before using the instrument, regardless of past NMR experience. Training is available for first time users. The facility is used by the Physical Chemistry and Forensic Chemistry classroom laboratories.
- No food or beverages near the spectrometer or workstation.
- Do not leave NMR samples in the NMR lab.
- If a sample breaks in the magnet or gets stuck in the magnet, contact the staff immediately so that the probe can be cleaned.
- All users must solvent lock on the dummy sample when finished using the spectrometer. The dummy sample is CDCl3. If the sample breaks or evaporates, contact the staff immediately so that the sample can be replaced.
- Prepare samples using standard 5 mm NMR tubes. The NMR lab does not provide samples tubes or solvents.
- The recommended minimum sample concentration and volume is 10 mg/mL and 0.5 mL.
- Sign up in ¼ hour increments using the scheduling calendar.
- Keep each user session to less than 1 hour between the hours of 8 am and 8 pm.
- Limit usage to 1 hour per user between noon and 6 pm Monday through Friday.
- Include a contact phone number or e-mail address when signing up to allow users to resolve scheduling conflicts.
- Do not sign up unless you are certain that you will be able to honor your commitment.
- Delete your name from the scheduling calendar as soon as possible when you determine that you cannot keep your time slot.
The schedule permits overnight runs (up to 12 h) from 8 pm to 8 am on weekdays; up to a 24 h run on the weekends starting Friday at 8 pm, and, on the 2nd weekend of each month, an extended run of up to 60 h beginning at 8 pm on Friday and ending by Monday at 8 am may be scheduled.
Users should schedule only a single 24 h experiment over the weekend (except for the second weekend of each month) to allow other users to use the instrument. Weekend time slots are for experiments that require very long data acquisition times and not for multiple short duration experiments.
There will be exceptions to these rules; if you need to use the instrument for 1-2 hours, you can do this once during the 8 am to 8 pm period every few weeks, but if you anticipate needing several sessions greater than 1 hour each, back-to-back sessions totaling more than one hour, and up to 12 hours during the course of a week, use the 8 pm to 8 am (overnight) period for this work.
The initial training session is intended to familiarize the user with running 1D experiments and usually takes about an hour to complete. Topics covered include: NMR sample preparation and handling. Set-up of a single nucleus 1D experiment and acquisition of a spectrum. Automatic and manual tuning and shimming Basic 1D processing and integration.
Directions for using and viewing the “Chemistry NMR400” scheduling calendar are at https://wiki.albany.edu/display/public/askit/Working+with+a+Room+Calendar Only users with an NMR account may access the calendar.
Dr. David S. Burz
Additional solution NMR spectrometers, used primarily for macromolecular structural studies, are part of the Life Science Research Building (LSRB) core facilities, which is located in LSRB 1166. These instruments include a HFCN, QCI-F, and quadruple resonance, HPCN, QCI-P cryoprobes; and a 700 MHz Bruker Avance II equipped with triple resonance, HCN, TXI cryoprobe. The 600 MHz NMR spectrometer is designed to support structural studies of nucleic acids.
Dr. Alex Shekhtman