The Facility provides the following services to investigators from any academic institution as well as corporate customers.
Affymetrix GeneChip Analysis
Affymetrix´s proprietary GeneChip technology utilizes photolithography based in–situ synthesis of arrays containing 25–mer oligonucleotide probes. We provide gene expression (3´–IVT, Gene ST and Transcriptome arrays), gene regulation (Tiling) and miRNA on the GeneChip cartridge platform. The core is experienced in working with small samples (LCM, biopsies, FFPE tissue, flow sorted cells and immunoprecipitated material)
Agilent microarray Analysis
Agilent´s proprietary SurePrint technology uses an inkjet, non–contact printing process to produce glass arrays containing 60–mer oligonucleotide probes.We provide gene expression (custom or whole genome), gene regulation (Promoter Tiling), miRNA, epigenetic pr ofiling (DNA Methylation) on the Agilent platform. The core is experienced in working with small samples (LCM, biopsies, flow sorted cells and immunoprecipitated material)
Custom microarray analysis
The core can also provide services on any custom arrays supplied by the end user. We have used many such slides spotted at other institutions for 1– or 2–color microarray analysis. Protein or cytokine arrays have also been scanned at our facility.
The core can provide services on qPCR arrays provided by SABiosciences/Qiagen.
RNA/DNA isolation and Quality Control
We can isolate total RNA, mRNA, small RNA, immunoprecipitated RNA and genomic DNA from a wide variety of samples including cell lines, eukaryotic tissues, prokaryotic cultures and plants. We also offer RNA, small RNA and DNA assays on the Agilent Bioanalyzer in order to check the integrity of your sample.
Data mining Services
The Core Facility has a variety of academic and commercial data mining software including GeneSpring (Agilent), Agilent Genome Workbench, Roche DEVA Expression Console and Transcriptome Analysis Console (Affymetrix).
Validation of expression analysis data
The facility offers a myriad of services for validation of gene expression data including SYBR Green and Taqman quantitative PCR assays.
For more information, we invite you to contact Dr Sridar Chittur, Director of the Microarray & HTS Core Facility