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Each sample should be provided in a single 1.5 mL microfuge tube with the initials of the Principle Investigator and a sample number written on the top.
- Purified RNA
RNA in water should be shipped on enough dry ice to cover the tubes entirely and with enough excess ice to allow for sublimation during shipping. RNA in ethanol should be well-sealed (snap cap with parafilm or screw cap) and can be shipped using gel ice packs or on dry ice.
- Purified DNA
Gel ice packs can be used for shipping.
- Tissue samples
Samples treated in RNAlater (Qiagen) can be shipped at room temperature. Otherwise, tissues should be collected with consideration given to the effects of RNAses – i.e. isolation or dissection should be done swiftly with RNAse-free instruments and collection tubes, then flash-frozen to -80°C. Samples thus collected can then be shipped to us on dry ice.
- Cell pellets
Cell pellets (washed with PBS) can be flash frozen to -80°C and shipped on dry ice, or the pelleted cells can be resuspended in Trizol (2 mL), then flash frozen and shipped on dry ice.For adherent cells, wash plate/flask with PBS, add Trizol directly to plate, coat the surface. The cells will slough off after 2 minutes. Pipette cell in Trizol to RNAse free tube, freeze and ship on dry ice.