Procedure for RNA Submission for labeling and/or hybridization
- For first time users, complete and submit a Customer Information Form that you can download in word or acrobat format.
- Contact Dr. Sridar Chittur at firstname.lastname@example.org to discuss the specifics of your project.
- After confirmation of project feasibility by e-mail, follow the RNA Quality Assurance Guidelines.
- Complete and submit the RNA submission sheet that you can download in word or acrobat format.
Each sample should be provided in a single 1.5 mL microfuge tube with the initials of the Principle Investigator and a sample number written on the top.
- Purified RNA
RNA in water should be shipped on enough dry ice to cover the tubes entirely and with enough excess ice to allow for sublimation during shipping. RNA in ethanol should be well-sealed (snap cap with parafilm or screw cap) and can be shipped using gel ice packs or on dry ice.
- Purified DNA
Gel ice packs can be used for shipping.
- Tissue samples
Samples treated in RNA later can be shipped at room temperature. Otherwise, tissues should be collected with consideration given to the effects of RNAses in mind - that is, isolation or dissection should be done swiftly with RNAse-free instruments and collection tubes , then flash-frozen to -80C. Samples collected thusly can then be shipped to us on dry ice.
- Cell pellets
Cell pellets (washed) can be flash frozen to -80C and shipped on dry ice, or the pelleted cells can be resuspended in Trizol (2 mL), then flash frozen and shipped on dry ice.