CFG Gene Targeting Services

Germline transmission of mouse chimaera

The CFG provides technologies for in vivo gene function analysis and generation of animal models produced via gene targeting in Embryonic Stem (ES) cells with subsequent blastocyst injections. This basic service is divided into two stages: Electroporation of ES cells and Blastocyst Injections. Both or either one of these services are offered at CFG.

Electroporation of Embryonic Stem Cells. This includes electroporationof DNA into ES cells, positive/negative selection, collection of 200 ES clones and screening. Individual clones with duplicates will be isolated for DNA analysis. Targeting will be in either JM-1 ES cells, derived from 129SvJ genetic background or W4 ES cells, derived from 129 SvEv.

Pricing

Please, contact us about pricing for Non-Academic institutions

Academic
$7,500

Additional Services

ServiceAcademic
Karyotyping $180
ES screening $1,500
ES expansion $150
Mycoplasma $150

To Request Services for Electroporation

  1. For first time users, complete and submit a Customer Information Form that you can download directly in either word or acrobatformats
  2. Complete and submit an ES Cell Form that you can download directly in either word or acrobat formats
  3. Complete and sumit the ES cell clone screening form that you can download directly in either word or acrobat formats
  4. Arrange to send DNA construct(s) to the CFG. Please refer to Instructions for DNA Cleaning and Shipping.

We recommend isolating the genomic DNA target from the same genetic background as the ES cells to be used. We offer two different genomic libraries to screen: a cosmid library derived from 129Sv and a lambda library derived from129 SvEv genetic background. For further information, regarding pricing, and our other customized services please contact Dr. Brian A. Parr at (518) 591-8307 or by e-mail: bparr@albany.edu.

Blastocyst Injections. ES cell clones that have been karyotyped will be injected into C57Bl6 blastocysts. Generation of at least 2 chimaeric mice is guaranteed. Mice are kept in SPF conditions within barrier facilities maintained by Taconic Farms, Inc.

Pricing (per single clone)

Please, contact us about pricing for Non-Academic institutions

Academic
$4,500

To Request Services for Blastocyst Injection

  1. For first time users, complete and submit a Customer Information Form that you can download directly in either word or acrobat formats.
  2. Complete and submit the Blastocyst Injection Form that you can download directly in either Word or Adobe formats. Please note that to complete this form you must have approval for the project from the animal welfare committee at your institution.
  3. Complete and submit the Mouse Screening Form that you can download directly in either Word or Acrobat formats.
  4. Complete and submit the Mouse Shipping Form that you can download directly in either Word or Adobe formats. Shipping will be arranged through Taconic Farms, Inc.

The investigator may send their own targeted ES cell clones for injection. However, we can not guarantee germline transmission for these clones.

For further information regarding pricing or to discuss customized services please contact Dr. Brian A. Parr at (518) 591-7213 or by e-mail: bparr@albany.edu.