Dr. Sridar Chittur

Sridar Chittur
Director, DNA Microarray Core Facilty
Research Associate Professor, Dept. Biomedical Sciences
School of Public Health

Academic Background

Professional Background

Honors and Awards

Research Interests

Research Experience

Utilization of Microarray and qPCR technologies for gene expression profiling and genotyping.
Utilization of Microarray and qPCR technologies for epigenetic profiling.
Utilization of multicolor flow cytometry for immunophenotyping and cell sorting.
Development of kinase inhibitors for pulmonary hypertension.
Investigation of an alternate mechanism of action for ansamycins.
Purification and characterization of human ADE2 protein overexpressed in E. coli.
Crystallization of IGP synthase, GMP synthetase and human ADE2.
Isolation and purification of wild type and mutant GMPS, IGPS and N¹-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide isomerase from E. coli.
Development of a viable expression system for human GMPS and the biophysical characterization of this enzyme from E. coli.
Synthesis of substrates and inhibitors to study the inhibition and mechanism of GMPS using stopped-flow spectroscopy.
Synthesis and characterization of inhibitors of IGPS from E. coli and S. cerevisiae
Synthesis of substrate analogs and inhibitors of glucosamine-6-phosphate synthase (involved in chitin biosynthesis) from C. albicans and S. cerevisiae.
Isolation and purification of glucosamine-6-phosphate synthase from C. albicans and S. cerevisiae
Development of a HPLC method to assay chlorzoxazone as a probe for cytochrome P450 2E1 metabolism.

Selected Publications (full list at Pubmed)

Dyavaiah, M. Rooney, J.P., Chittur, S.V., Lin, Q. Begley, T.J. (2011)
Autophagy-Dependent Regulation of the DNA Damage Response Protein Ribonucleotide Reductase 1.
Mol. Can. Research February 22

Jain R., Devine T., George A.D., Chittur S.V., Baroni T.E., Penalva L.O., Tenenbaum S.A.(2011)
RIP-Chip analysis: RNA-Binding Protein Immunoprecipitation-Microarray (Chip) Profiling.
Methods in Molecular Biology 703, 247-263.

Gorski J.P., Huffman, N.T., Chittur S.V., Midura R.J., Black, C. Oxford, J, Seidah, N.G. (2011)
Inhibition of SKI-1 proprotein convertase and caspase-3 blocks transcription of key extracellular matrix genes regulating osteoblastic mineralization.
J. Biol Chem. 286(3), 1836-1849.

Gibbs, K., Chittur, S.V., Szaro, B.G. (2010)
The loss of regenerative capacity of spinal cord axons resulting from metamorphosis in Xenopus laevis
Eur. J. Neuroscience 1-17.

Avasarala, J.R, Chittur, S.V., Kuentzel, M, Frank, D. (2010)
Microarray and miRNA analysis of brain lesions in African-American and Caucasians with multiple sclerosis.
Internet J. Neurology 13(1).

Subbaram, S. Kuentzel M, Frank, D, DiPersio C.M., Chittur S.V. (2010)
Determination of Alternative Splicing Events on the Affymetrix Exon 1.0 ST Arrays.
Microarrays in Drug Discovery. 632, 63-72.

Chittur S.V. (2010), Editor
Microarrays in Drug Discovery, Methods in Mol. Biol v632 (Humana Press)

Guda, P., Chittur, S.V., Guda, C. (2009)
Comparative analysis of protein-protein interactions in cancer-associated genes
Genomics, Proteomics and Bioinformatics 7, 25-36

Oyama, M.A, Chittur, S.V., Reynolds C.A. (2009)
Decreased triadin and increased calbstatin2 expression in great danes with dilated cardiomyopathy
J. Vet . Intern. Med 23(5), 1014-1019

Chittur S. V., Sangster-Guity, N., McCormick, P.J. (2008)
Histone deacetylase inhibitors: A new mode for inhibition of cholesterol metabolism
BMC Genomics 9, 507

Society Memberships