Sridar Chittur
Director, DNA Microarray Core Facilty
Adjunct Assistant Professor, Biomedical Sciences
Academic Background
- Ph.D. (Medicinal Chemistry) 1996, West Virginia University, Morgantown, WV
- B.S. (Pharmacy) 1991, Bombay University, India.
Professional Background
- Senior Scientist, Myomatrix Therapeutics, Rensselaer, NY (2002)
- Post-doctoral Fellow, Purdue University, West Lafayette, IN (1997-2002)
Honors and Awards
- Rho Chi Pharmacy Honor Society
Research Experience
- Utilization of Affymetrix GeneChip technology and cDNA spotted arrays for gene expression profiling.
- Utilization of multicolor flow cytometry for immunophenotyping and cell sorting.
- Investigation of an alternate mechanism of action for ansamycins.
- Purification and characterization of human ADE2 protein overexpressed in E. coli.
- Crystallization of IGP synthase, GMP synthetase and human ADE2.
- Isolation and purification of wild type and mutant guanosine monophosphate synthetase, imidazole glycerol phosphate synthase and N1-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide isomerase from E. coli.
- Development of a viable expression system for human guanosine monophosphate synthetase and the biophysical characterization of this enzyme from E. coli.
- Synthesis of various compounds to study the inhibition and mechanism of GMPS using stopped-flow spectroscopy.
- Synthesis and characterization of inhibitors of imidazole glycerol phosphate synthase from E. coli and S. cerevisiae
- Synthesis of substrate analogs and inhibitors of glucosamine-6-phosphate synthase (involved in chitin biosynthesis) from C. albicans and S. cerevisiae.
- Isolation and purification of glucosamine-6-phosphate synthase from C. albicans and S. cerevisiae
- Development of a HPLC method to assay chlorzoxazone as a probe for cytochrome P450 2E1 metabolism.
Selected Publications
- Oyama, M.A and Chittur, S. (2005) Genomic expression profiling of canine dilated cardiomyopathy., American Journal of Veterinary Research (accepted, in press)
- Eifert, C., Sangster-Guity, N., Yu, L-M, Chittur, S.V., Perez, A.V., Tine, J.A. and McCormick, P.J. (2005) Global gene expression profiles associated with retinoic acid-induced differentiation of embroyonal carcinoma cells. Molecular Reproduction & Development (accepted, in press)
- Chittur S.V. (2005) DNA Microarrays. A comprehensive review, Encyclopedia of Medical Genomics & Proteomics (accepted, in press)
- Chittur S.V. (2004) DNA Microarrays: Tools of the 21st Century, Combinatorial Chemistry & High Throughput Screening, 7, 531-537.
- Boldyrev A.A., Kazey V.I., Leinsoo T.A., Mashkina A.P., Tyulina O.V., Johnson, P. Tuneva, J.O., Chittur, S., Carpenter, D.O., (2004) Rodent lymphocytes express functionally active glutamate receptors., Biochemical and Biophysical Research Communications, 324, 133-139
- Chittur S.V. and Griffith R.K., (2002). Multisubstrate analogue inhibitors of glucosamine-6-phosphate synthase from C. albicans, Bioorganic and Medicinal Chemistry Letters, 12, 2639-2642.
- Chittur S.V., Klem T.J., Shafer C.M., Davisson V.J. (2001). Mechanism for acivicin inactivation of triad glutamine amidotransferases, Biochemistry, 40 (4); 876-887.
- Chaudhuri, N., Lange, S., Myers R.S., Chittur S.V., Sainz, G., Davisson V.J., Smith J.L., (2001). Crystal Structure of imidazole glycerol phosphate synthase: A tunnel through a (β/α)8 barrel joins two active sites., Structure, 9 (10); 987-997.
- Chittur S.V., Chen Y, Davisson V.J. (2000). Expression and purification of IGP synthase from S. cerevisiae , Protein Expression and Purification, 18 (3), 366-377.
- Deras M.L., Chittur S.V., Davisson V.J. (1999). Hydroxylamine inhibition of E. coli guanosine monophosphate synthetase: N2-OH-GMP is a time dependent inhibitor, Biochemistry, 38 (1), 303-310.
- Chittur S.V., Tracy T.S. (1997). A rapid and sensitive high performance liquid chromatographic assay for 6-hydroxychlorzoxazone and chlorzoxazone in liver microsomes, Journal of Chromatography B, 693, 479-483.
- Griffith R.K., Chittur S.V., Chen Y.C. (1992). Inhibition of glucosamine-6-phosphate synthase from C. albicans by quinoxaline-2,3-diones, Medicinal Chemistry Research, 2, 536-547.
In Development
- Chittur S.V., Eifert C., Devine T., Kuentzel M. and McCormick, P.J. (2005) Determination of normal age, gender and tissue variance in an inbred strain of mice. Mammalian Genome (in review)
- Di-Marco S, Mazroui R., Dallaire P., Chittur S., Tenenbaum S., Radzioch, D., Gallouzi I-E., (2005). Nitric oxide induced muscle wasting requires the stabilization of iNOS mRNA by HuR protein. (in review).
- Chittur S.V., Eifert C., Devine T., Kuentzel M. and McCormick, P.J. Determination of normal variance in brain, liver and kidney between three inbred strains of mice. (In preparation)
- Chittur S.V. and Davisson V.J., Inhibition of SAICAR synthetase: An alternate mechanism for ansamycin anticancer activity, (in preparation)
- Oliver J.C., Chittur S.V., Burgner J.W., Davisson V.J.,. Kinetic Mechanism of guanosine monophosphate synthetase, (in preparation)