Joseph Wade

  • Joseph Wade


    Dr. Wade’s laboratory focuses on the regulation of gene expression in bacteria. Specifically, he studies the mechanisms of transcriptional regulation in Escherichia coli K-12, and pathogenic E. coli, Salmonella enterica and Yersinia pestis. There are three major research focuses: (i) regulation of transcription by DNA-binding proteins, (ii) the role of non-coding RNAs in regulation of gene expression, and (iii) regulation of ribosomal RNA transcription and processing. The laboratory uses a combination of genetic, biochemical and genomic approaches. In particular, Dr. Wade has pioneered the use of ChIP-chip and ChIP-seq in bacteria to map the binding sites of transcription factors genome-wide. Dr. Wade has also pioneered the use of RNA-seq to map transcription start sites in bacteria. His work using this and other genomic methods has led to fundamental discoveries relating to the regulation of gene expression. For example, the laboratory has recently identified thousands of non-coding RNAs in E. coli that are transcribed from within genes. The laboratory (in collaboration with Dr. Kathleen McDonough’s laboratory) has also identified many non-coding RNAs in Y. pestis that are likely to be involved in various aspects of pathogenesis.