Features of Husbandry Equated Across Labs, cont�d
Mated pairs (items the same as after arrival are not repeated)
Food Purina 5020 (9% fat)
Nesting material 1 Nestlet
Marking none
Weighing at mating
Mice/cage 1 female, 1 male
Male present with litter? Yes; postpartum pregnancy allowed
Inspection of new litter Count live and dead pups; remove dead, put live in nest
Culling of newborns Remove only the obvious runts for ethanasia
Cage changing Weekly
Cage changing with litter Do not disturb cage until litter is 4 days old or younger; then clean cage with fresh nestlet