Alexander Shekhtman


Biophysics | Spin nuclear physics


  • Kucirka, J and Shekhtman A. 1996. On the deracemization of a chiral molecular beam by interaction with circularly polarized light. Physics Letters A221, 273-276.
  • Shekhtman A, McNaughton L, Cunningham RP, and Baxter SM. 1999. Identification of the Archaeoglobus fulgidus endonuclease III-DNA interaction surface using heteronuclear NMR methods. Structure with Folding and Design, 7, 919-30.
  • Begley TJ, Haas BJ, Shekhtman A, Williams WA, and Cunningham RP. 1999. A new member of the endonuclease III family of DNA repair enzymes, which removes methylated purines from DNA. Current Biology 9, 653-656.
  • Ghose R*, Shekhtman A*, Goger MJ, Ji H, and Cowburn D. 2001. A novel, specific interaction involving the Csk SH3 domain and its natural ligand. Nature Structural Biology 8, 998-1004.
  • Shekhtman A*, Ghose R*, Wang D, Cole PA, and Cowburn D. 2001. Novel mechanism of regulation of the non-receptor protein tyrosine kinase Csk: insights from NMR mapping studies and site-directed mutagenesis. Journal of Molecular Biology 314, 129-38.
  • Camarero JA*, Shekhtman A*, Campbell EA, Chlenov M, Gruber TM, Bryant DA, Darst SA, Cowburn D, and Muir TW. 2002. Autoregulation of a bacterial sigma factor explored by using segmental isotopic labeling and NMR. Proceedings of the Natural Academy of Sciences U S A 99, 8536-41. Reviewed in Faculty of 1000 Biology.
  • Shekhtman A, Ghose R, Goger M, and Cowburn D. 2002. NMR structure determination and investigation using a reduced proton (REDPRO) labeling strategy for proteins. FEBS Letters 524, 177-82.
  • Shekhtman A and Cowburn D. 2002. A ubiquitin-interacting motif from Hrs binds to and occludes the ubiquitin surface necessary for polyubiquitination in monoubiquitinated proteins. Biochemical and Biophysical Research Communications 296, 1222-7.
  • Romanelli A, Shekhtman A, Cowburn D, and Muir TW. 2004. Semisynthesis of a segmental isotopically labeled protein splicing precursor: NMR evidence for an unusual peptide bond at the N-extein-intein junction. Proceedings of the Natural Academy of Sciences U S A 101, 6397-402
  • Cowburn D, Shekhtman A, Xu R, Ottesen JJ, and Muir TW. 2004. Segmental isotopic labeling for structural biological applications of NMR. Methods Mol Biol. 278, 47-56
  • Shekhtman A. 2005. Protein Chemical Ligation as an Invaluable Tool for Structural NMR. Protein and Peptide Letters 12, 765-768.
  • Ji H, Shekhtman A, Ghose R, McDonnell JM, and Cowburn D. 2006. NMR determination that an extended BH3 motif of pro-apoptotic BID is specifically bound to BCL-xL. Magn. Reson. Chem. 44
  • Burz DS, Dutta K, Cowburn D, and Shekhtman A. 2006. Mapping structural interactions using in-cell NMR spectroscopy (STINT-NMR). Nature Methods 3, 91-3. Reviewed in Faculty of 1000 Biology and in Selenko P and Wagner G. NMR mapping of protein interactions in living cells. 2006. Nat Methods. 3, 80-1.
  • Burz DS, Dutta K, Cowburn D, and Shekhtman A. 2006. STINT-NMR (in-cell NMR for protein-protein interactions). Nature Protocols. 1, 146-52.
  • Xie J, Burz DS, He W, Bronstein IB, Lednev I, and Shekhtman A. 2007. Hexameric calgranulin C (S100A12) binds to the receptor for advanced glycated end products (RAGE) using symmetric hydrophobic target-binding patches. J Biol Chem. 282, 4218-31.
  • Camarero JA, Kimura RH, Woo YH, Shekhtman A, and Cantor J. 2007. Biosynthesis of a fully functional cyclotide inside living bacterial cells. Chembiochem. 8, 1363-6.
  • Liu Q, Dansereau JT, Puttamadappa SS, Shekhtman A, Derbyshire V, and Belfort M. 2008. Role of the interdomain linker in distance determination for remote cleavage by homing endonuclease I-TevI. J Mol Biol. 379, 1094-106.
  • Burz DS and Shekhtman A. 2008. In-cell Biochemistry using NMR spectroscopy. PloS One. 3(7):e2571.
  • Xie J, Reverdatto S, Frolov A, Hoffmann R, Burz DS, Shekhtman A. 2008. Structural basis for pattern recognition by the receptor for advanced glycation end products (RAGE). J Biol Chem. Jul 30. 283, 27255-27269
  • Schwartz EC, Shekhtman A, Dutta K, Pratt MR, Cowburn D, Darst S, Muir TW.2008. A full-length group 1 bacterial sigma factor adopts a compact structure incompatible with DNA binding. Chem Biol. 15, 1091-103. Reviewed in Faculty of 1000 Biology.
  • Pande A, Zhang J, Banerjee PR, Puttamadappa SS, Shekhtman A, Pande J. NMR study of the cataract-linked P23T mutant of human gammaD-crystallin shows minor changes in hydrophobic patches that reflect its retrograde solubility. 2009. Biochem Biophys Res Commun. 382, 196-9.
  • Burz DS, Shekhtman A. 2009. Inside the living cell. Nature 458, 37-38.
  • Moroz O, Burkitt W, Wittkowski H, Ianoul A, Xie J, Polyakova O, Lednev IK, Shekhtman A, Bjoerk P, Bronstein IB. 2009. Both Ca2+ and Zn2+ are essential for S100A12 protein -oligomerization and function. BMC Biochemistry, 10:11, doi:10.1186/1471-2091-10-11.
  • Huang Z, Pei W, Han Y, Jayaseelan S, Shekhtman A, Shi H, Niu L. 2009. One RNA aptamer sequence, two structures: a collaborating pair that inhibits AMPA receptors, Nucleic Acids Research. 12, 4022. Reviewed in Faculty of 1000 Biology:
  • Xie J, Thapa R, Burz DS, Reverdatto S, Shekhtman A. 2009. Screening of Small Molecule Interactor Library by using In-Cell NMR spectroscopy (SMILI-NMR). Journal of Medicinal Chemistry, 52, 3516-22. Highlighted in Nature Chemical Biology 2009, 5, 457. Reviewed in Faculty of 1000 Biology:

Spin nuclear physics

  • Baryshevsky VG, Tikhomirov VV, and Shekhtman A. 1989. A new mechanism of absorption anisotropy of high energy gamma-rays in a crystal. Sov. Technical Physics Letters 15, 154-157
  • Baryshevsky VG, Tikhomirov VV, and Shekhtman A. 1991. How diffraction influences absorption anisotropy of high energy g-rays incident under small (microradiant) angles to the crystal axes. Byelorussian Acad. Reports N2, 39-48
  • Shekhtman A. 1991. Anomalous magnetic moment of high-energy electrons and positrons moving in a crystal and in an amorphous medium. Physics Letters A160, 575-578.
  • Baryshevsky VG and Shekhtman A. 1992. On the possibility of measurement of omega-minus -hyperon quadrupole moment in crystals. 1992. Byelorussian Acad. Reports N3, 87-95
  • Shekhtman A and Baryshevsky VG. 1993. Spin oscillation and the possibility of quadrupole moment measurement for omega-minus hyperon moving in a crystal. Nuclear Instruments and Methods in Physics Research B83, 1250-1255.
  • Baryshevsky VG and Shekhtman A. 1994. Proton (neutron) spin rotation in polarized nuclear target: a new technique for investigating exotic baryon states. Physics of Atomic Nuclei 57, 1299-1307
  • Shekhtman A, Tikhomirov VV, and Baryshevsky VG. 1994. A new mechanism of absorption anisotropy of high energy photons in a crystal. Nuclear Physics B424, 418-428.
  • Shekhtman A and Baryshevsky VG. 1996. Proton (neutron) spin rotation in polarized nuclear target: a new method for investigating nuclear interactions. Physical Review C53, 267-276