Raman Microscope

Access or consultation: Dr. Vladimir Ermolenkov, 591-8890 or vermolenkov@albany.edu

Raman spectroscopy is an effective method of chemical analysis and the study of the composition and structure of substances. In the case of Raman scattering, new spectral bands which are not present in the spectrum of the exciting light appear in the spectrum of the radiation scattered by the substance. The frequencies of the new bands are shifted up or down relative to the frequency of the exciting radiation. The shifts give the information about the vibrational modes in the studied system. The number, position, intensity, and polarization of the shifted bands are determined by the molecular structure of the substance.

Raman spectroscopy is used extensively in chemistry for the identification of substances, determination of the individual chemical bonds and groups in the molecules, study intra- and intermolecular interactions, different types of isomerism, phase transitions, and hydrogen bonding.

Because Raman spectroscopy is a sensitive and non-destructive technique, it can be used in forensic applications for detection and analysis of race amounts of a substance without destroying the sample.

In biology and medicine, Raman spectroscopy can be applied for study of structure of proteins, polypeptides, nucleic acids, lipids, oligosaccharides, and biotissues.

InVia Raman microscope (Renishaw, Inc) can be used for obtaining Raman spectra of various liquid, gelatinous, solid, and powder samples. Combination of three lasers allows to cover the wide range of Raman excitation wavelengths in visible – near IR spectral region. Available excitation wavelengths are 406.7, 457, 488, 514, 647, and 785 nm. The wide-range scanning XYZ stage provides the possibility of Raman spectra measuring in different points of the sample with subsequent creation of Raman maps.

The Raman microscope is located in the room LS 2068.

View the brochure here.