Beckman-Coulter Optima Ultracentrifuges

For access or consultation: Dr. Kim DeWeerd 591-8831 or kdeweerd@albany.edu

The Molecular Core Facility has three Optima Ultracentrifuges. Two floor models: Optima LE-80K, Optima L-90K and one tabletop model; Optima TLX.

Purpose:
Ultracentrifugation is a process where samples are spun at extremely high speeds to create gravitational forces (g force) great enough to separate membranes from soluble cell components, isolate organelles, and run gradients for separating DNA molecules, RNA and small cellular particles.

Specific Description:
These floor model ultracentrifuges are used for high speed centrifugation. Volumes from less than 0.5 milliliters up to 472 milliliters can be prepared in these centrifuges depending on the rotor used.

Available Rotors in the Molecular Core Facility

Fixed Angle Rotors

Rotor Max (rpm) Max (g force) Tube Volume Capacity
60Ti 60,000 485,000 4 ml 6
70Ti 70,000 361,000 39 ml 8
90Ti 90,000 503,000 13.5 ml 8
TLA-120.1 120,000 513,000 0.5 ml 14
TLA 120.2 120,000 513,000 1 ml 10
VTi 65.1 65,000 363,000 13.5 ml 8
VTi 65.2 65,000 385,000 5.1 ml 16

Swinging Bucket Rotors

SW 28 28,000 107,000 38.5 ml 6
SW 40Ti 40,000 202,000 14 ml 6
SW-41Ti 41,000 207,000 13.2 ml 6
TLS-55 55,000 201,000 2.2 ml 4

For More Information:
Optima LE-80K, L-90K brochure
Optima LE-80K Operation Manual
Optima TLX Operation Manual

Instrument Photographs:
click to enlarge