Biological Imaging

Digital Microscopy and Digital Imaging

The Biological Imaging Facility maintains excellent optics for a variety of imaging applications.

In addition to confocal imaging, two methods for digital capture are provided within the Facility:

  • The Zeiss LSM510 provides scanning lasers and a photomultiplier tube for capturing transmitted or DIC images on either inverted or upright microscopes;
  • A High-Performance Quantitative CCD Camera and its associated computer provide high sensitivity monochrome imaging with 12-bit, low-noise output at 1360 x 1036 pixel resolution. Full resolution acquisition can occur at 5 fps. The camera is currently configured for control through one of three software packages:
    • Using its own driver for adjustment and simple frame grabs
    • Using SimplePCI from Compix (for instances where camera control requires more features including control of the microscope, time-lapse imaging, and other image processing functions)
    • Using ImageJ (for those familiar with the NIH/Scion Image software)

Throughout the Department, video imaging systems based on MetaMorph (Universal Imaging), Image (NIH/Scion) and Hamamatsu are used. Because of their integration with other experimental equipment, they are typically in individual research labs.

High quality imaging of Calcium-sensitive fluorescence (MetaFluor, Universal Imaging) is available on an Olympus inverted microscope.