Publications: Marlene Belfort

  • Xie, J., Du, Z., Callahan, B., Belfort, M. and Wang, C.  1H, 13C, and 15N NMR assignments of a Drosophila Hedgehog autoprocessing domain.  Anal. Chem. (2013) 85, 6080-6088.
  • Contreras, L.M., Huang, T., Piazza, CL, Smith, D., Qu, Guosheng, G., Gelderman, G., Potratz, J., Russell, R. and Belfort, M.  Group II intron-ribosome association protects intron RNA from degradation.  Submitted.
  • Sorci, M., Liu,H, Anand, G., Dutta, A. Belfort, M., Pietrokovki, S. and Belfort, G.  Intermolecular force measurements indicate compatibility of cyanbacterial trans-splicing intein segments by a zipper-like mechanism.  Analytical Biochemistry (2013).  In Press.
  • Belfort, M. and Bonocora, R.  Homing endonucleases:  from genetic anomalies to programmable genomic scissors.  Homing Endonucleases, Methods in Molecular Biology (2013).  In press.
  • Bonocora, R. and Belfort, M.  Mapping homing endonuclease cleavage sites using in vitro generated protein.  Homing Endonucleases, Methods in Molecular Biology (2013).  In press.
  • Jaeger, J. and Belfort, M.  Hand-holding for retrohoming in a molecular diversity dance. Structure (2013).  In press.
  • Dearden, A., Callahan, B., Van Roey, P., Li, Z., Kumar, U., Belfort, M. and Nayak, S.K.  A conserved threonine spring-loads precursor for intein splicing.  Protein Science (2013) 22, 557-563.
  • Callahan, B., Stanger, M. and Belfort, M.  A redox trap to augment the intein toolbox.  Biotech. and Bioeng.(2013) 110,  1565-1573.
  • Edgell, D.R., Chalamcharla, V.R. and Belfort, M.  Learning to live together: mutualism between self-splicing introns and host genomes.  BMC Biology (2011) 9, 22. 
  • Belfort, M., Curcio, M.J., and Lue, N.F.  Telomerase and retrotransposons:  Reverse transcriptases that shaped genomes.   Proc. Natl. Acad. Sci. USA (2011) 108, 20304-20310.
  • Shemella, P.T., Soga, I., Pereira, B., Belfort, G., Belfort, M. and Nayak, S.K. Electronic structure of neighboring extein sequence modulates intein C-terminal cleavage activity. Biophys. J. (2011) 100, 2217-2225. 
  • Callahan, B.P., Topilina, N., Stanger, M.J, Van Roey, P and Belfort, M. Catalytically competent intein caught in a redox trap: Structure, function, evolution. Nature Struc. Mol. Biol. (2011) 18, 630-633..
  • Robbins, J.B., Smith, D and Belfort, M. Redox-responsive zinc-finger is fidelity switch in homing endonuclease, promoting intron promiscuity under oxidative stress. Current Biol.  (2011) 21, 243-248. 
  • Pereira, B., Shemella, P.T., Amitai, G., Belfort, G., Nayak, S.K. and Belfort, M. Spontaneous proton transfer to a conserved intein residue determines on-pathway protein splicing. J. Mol. Biol. (2010) 406, 430-442
  • Huang, T, Shaikh, T., Gupta, K., Contreras-Martin, L., Grassucci, R.A., Van Duyne, G.D., Frank, J.and Belfort, M. The group II intron ribonucleoprotein precursor is a large, loosely packed structure. Nucleic Acids Res. (2011) 39, 2845-2854.  Featured article (top 5%)
  • DiChiara, J.M., Contreras-Martinez, L.M., Livny, J., Smith, D., McDonough, K.A. and Belfort, M. Multiple small RNAs identified in Mycobacterium bovis BCG are also expressed in M. tuberculosis and M. smegmatis. Nucleic Acids Res. (2010) 38, 4067-4078.
  • Chalamcharla, V.R., Curcio, M.J. and Belfort, M. Nuclear expression of a group II intron is consistent with spliceosomal intron ancestry. Genes Dev. (2010) 24, 827-836. (Faculty of 1000, FFa10)
  • Du, Z., Liu, Y., Ban, D., Lopez, M.M., Belfort, M. and Wang, C. Backbone dynamics and global effects of an activating mutation in minimized Mtu RecA inteins. J. Mol. Biol. (2010) 400, 755-767.
  • Stoddard, B. and Belfort, M. Social networking between mobile introns and their host genes. Mol. Micro. (2010) 78, 1-4.
  • Edgell, D.R., Gibb, E.A., Shub, D.A. and Belfort, M. Mobile DNA elements in T-even phage. BMC Virology J. (2010) 7, 290-305.
  • Callahan, B.P., Stanger, M.J. and Belfort, M. Protease activation of green fluorescent protein. Chembiochem. (2010) 11, 2259-2263.
  • Zhang, L., Zheng, Y., Callahan, B., Belfort, M. and Liu, Y. Cisplatin inhibits protein splicing suggesting inteins as therapeutic targets in mycobacteria. J. Biol. Chem. (2010) 286, 1277-1282. Highlighted in Nature Chemical Biology.
  • Coros, C.J., Piazza, C. L., Chalamcharla, V.R., Smith, D. and Belfort, M. Global regulators orchestrate group II intron retromobility. Mol. Cell (2009) 34, 250-256. (Faculty of 1000, FFa8)
  • Amitai, G., Callahan, B.P., Stanger, M.J., Belfort, G. and Belfort, M. Modulation of intein activity by its neighboring extein substrates. Proc. Natl. Acad. Sci. USA (2009) 106, 11005-11010.
  • Du, Z., Shemella, P.T., Liu, Y., McCallum, S.A., Pereira, B., Nayak, S.K., Belfort, G., Belfort, M. and Wang, C. Highly conserved histidine plays a dual catalytic role in protein splicing: a pKa shift mechanism. J. Am. Chem. Soc. (2009) 131, 11581-11589.
  • Hiraga, K., Soga, I., Dansereau, J.T., Pereira, B., Derbyshire, V., Du, Z., Wang, C., Van Roey, P., Belfort, G. and Belfort, M. Selection and structure of hyperactive inteins: peripheral changes relayed to the catalytic center. J. Mol. Biol. (2009) 393, 1106-1117.
  • Belfort, M. Scientific serendipity initiates an intron odyssey. J. Biol. Chem. (2009) 284, 29997-30003.
  • Du, Z., Liu, Y., Zheng, Y., McCallum, S., Dansereau, J., Derbyshire, V., Belfort, M., Belfort, G., Van Roey, P. and Wang, C. 1H, 13C, and 15N NMR assignments of an engineered intein based on Mycobacterium tuberculosis RecA. Biomol. NMR Assign (2008) 2, 111-113.
  • Liu, Q.Q., Dansereau, J.T., Puttamadappa, S,S, Shekhtman, A., Derbyshire, V. and Belfort, M. Role of the interdomain linker in distance determination for remote cleavage by homing endonuclease I-TevI . J. Mol. Biol. (2008) 379, 1094-1106.
  • Beauregard, A., Curcio, M.J. and Belfort, M. The take and give between retrotransposable elements and their hosts. Annu. Rev. Genetics. (2008) 42, 587-617.
  • Coros, C.J., Piazza, C. L. , Chalamcharla, V.R. and Belfort, M. A mutant screen reveals RNase E as a silencer of group II intron retromobility in Escherichia coli. RNA (2008) 14, 2634-2644.
  • Van Roey, P., Pereira, B., Li, Zh., Hiraga, K., Belfort, M., and Derbyshire, V. Crystallographic and mutational studies of Mycobacterium tuberculosis recA mini-inteins suggest a pivotal role for a highly conserved aspartate residue. J. Mol. Biol. (2007) 367, 162-173.
  • Slagter-Jäger, J.G., Puzis, L., Gutgsell, N.S., Belfort, M. and Chaitanya, J. Functional defects in transfer RNAs lead to the accumulation of ribosomal RNA precursors. RNA (2007) 13, 597-605.
  • Robbins, J.B., Stapleton, M., Stanger, M.J., Smith, D., Dansereau, J.T., Derbyshire, V., and Belfort, M. Homing endonuclease I-TevIII: dimerization as a means to a double-strand break. Nucleic Acids Res. (2007) 35, 1589-1600.
  • Curcio, M.J. and Belfort, M. The beginning of the end: Links between ancient retroelements and modern telomerases. Proc. Natl. Acad. Sci. USA (2007) 104, 9107-9108.
  • Liu, Q.Q., Derbyshire, V., Belfort, M. and Edgell, D.R. Distance determination by GIY-YIG intron endonucleases: Discrimination between repression and cleavage functions. Nucleic Acids Res. (2006) 34, 1755-1764.
  • Slagter-Jäger, J.G., Allen, G.S., Smith, D., Hahn, I.A., Frank, J. and Belfort, M. Visualization of a group II intron in the 23S rRNA of a stable ribosome. Proc. Natl. Acad. Sci. USA. (2006) 103, 9838- 9843.
  • Silva, G.H., Belfort, M., Wende, W. and Pingoud, A. From Monomeric to Homodimeric Endonucleases and Back: Engineering Novel Specificity of LAGLIDADG Enzymes. J. Mol. Biol. (2006) 361, 744-754.
  • Beauregard, A., Chalamcharla, V.R., Piazza, C.L., Belfort, M. and Coros, C.J. Bipolar localization of the group II intronLl.LtrB is maintained in Escherichia coli deficient in nucleoid condensation, chromosome partitioning and DNA replication. Mol. Micro. (2006) 62, 709-722.
  • Blocker, F.J.H., Mohr, G., Conlan, L.H., Qi, L., Belfort, M. and Lambowitz, A.M. Domain structure and three-dimensional model of a group II intron-encoded reverse transcriptase. RNA (2005) 11, 14-28.
  • Miao, J., Wu, W., Spielmann, T., Belfort, M., Derbyshire, V., and Belfort, G. Single-step affinity purification of toxic and non-toxic proteins on a fluidics platform. Lab on a Chip (2005) 5, 248-253.
  • Van Roey, P., Belfort, M., Derbyshire, V. Homing endonuclease I-TevI: an atypical zinc finger with a novel function. In: Iuchi S, Kuldell N, eds. Zinc finger proteins: from atomic contact to cellular function. Georgetown: Landes Bioscience, New York: Kluwer Academic / Plenum Publishers (2005) 35-38.
  • Coros, C.J., Landthaler, M., Piazza, C.L., Beauregard, A., Esposito, D., Perutka, J., Lambowitz, A.M. and Belfort, M. Retrotransposition strategies of the Lactococcus lactis Ll.LtrB group II intron are dictated by host identity and cellular environment. Mol. Microbiol. (2005) 56, 509-524.
  • Belfort, M. Back to basics: structure, function, evolution and application of homing endonucleases and inteins, in Homing Endonucleases and Inteins, Eds. Belfort, M., Derbyshire, V., Stoddard, B.L. and Wood, D.W., Springer-Verlag (2005) pp. 1-10.
  • Smith, D., Zhong, J., Matsuura, M., Lambowitz, A. M. and Belfort, M. Recruitment of host functions suggests a repair pathway for late steps in group II intron retrohoming. Genes Dev. (2005) 19, 2477-2487.
  • Conlan, L. H., Stanger, M. J., Ichiyanagi, K. and Belfort, M. Localization, mobility and fidelity of retrotransposed group II introns in rRNA genes. Nucl. Acids Res. (2005) 33, 5262-5270.
  • Hiraga, K., Derbyshire, V., Dansereau, J. T., Van Roey, P. and Belfort, M. Minimization and stabilization of the Mycobacterium tuberculosis recA intein. J. Mol. Biol. (2005) 354, 916-926.
  • Silva, G.H. and Belfort, M. Analysis of the LAGLIDADG interface of the monomeric homing endonuclease I-DmoI. Nucleic Acids Res. (2004) 32, 3156-3168.
  • Lease, R.A., Smith, D., McDonough, K., and Belfort, M. The small non-coding DsrA RNA is an acid resistance regulator in Escherichia coli. J. Bacteriol. (2004) 186, 6179-6185.
  • Edgell, D.R., Derbyshire, V., Van Roey, P., LaBonne, S., Stanger, M.J., Li, Z., Boyd, T.M., Shub, D.A., and Belfort, M. Intron-encoded homing endonuclease I-TevI also functions as a transcriptional autorepressor. Nat. Struct. Mol. Biol. (2004) 11, 936-944.
  • Edgell, D.R., Stanger, M.J., and Belfort, M. Coincidence of cleavage sites of intron endonuclease I-TevI and critical sequences of the host thymidylate synthase gene. J. Mol. Biol. (2004) 343, 1231-1241.
  • Roberts, R.J., Belfort, M. et al. A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes. Nucleic Acids Res. (2003) 31, 1805-1812.
  • Edgell, D.R., Stanger, M.J. and M. Belfort. Importance of a single base pair for discrimination between intron-containing and intronless alleles by endonuclease I-BmoI. Curr. Biol. (2003) 13, 973-978.
  • Liu, Q., Belle, A., Shub, D.A., Belfort, M. and Edgell, D.R. SegG endonuclease promotes marker exclusion and mediates co-conversion from a distant cleavage site. J. Mol. Biol. (2003) 334, 13-23.
  • Ichiyanagi, K., Beauregard, A. and Belfort, M. A bacterial group II intron favors retrotransposition into plasmid targets. Proc. Natl. Acad. Sci. USA (2003) 100, 15742-15747.
  • Belfort, M. Two for the price of one: a bifunctional intron-encoded DNA endonuclease-RNA maturase. Genes Dev. (2003) 17, 2860-2863.
  • Belfort, M., Derbyshire, V., Parker, M.M., Cousineau, B. and Lambowitz, A.M. Mobile introns: pathways and proteins. In Mobile DNA II. (2002) N. Craig, R. Craigie, M. Gellert and A. Lambowitz, eds. ASM Press. 761-783.
  • Dean, A.B., Stanger, M.J., Dansereau, J.T., Van Roey, P., Derbyshire, V., and Belfort, M. Zinc finger as distance determinant in the flexible linker of intron endonuclease I-TevI. Proc. Natl. Acad. Sci. USA (2002) 99, 8554-8561.
  • Ichiyanagi, K., Beauregard, A., Lawrence, S., Smith, D., Cousineau, B. and Belfort, M. Retrotransposition of the Ll.LtrB group II intron proceeds predominantly via reverse splicing into DNA targets. Mol. Micro. (2002) 46, 1259-1272.
  • Van Roey, P., Meehan, L., Kowalski, J.C., Belfort, M. and Derbyshire, V. Catalytic domain structure and hypothesis for function of GIY-YIG intron endonuclease I-TevI. Nat. Struct. Biol. (2002) 9, 806-811.
  • Wu, W., Wood, D.W., Belfort, G., Derbyshire, V. and Belfort, M. Intein-mediated purification of cytotoxic endonuclease I-TevI by insertional inactivation and pH-controllable splicing. Nucleic Acids Res. (2002) 30, 4864-4871.
  • Van Roey, P., Waddling, C.A., Fox, K.M., Belfort, M. and Derbyshire, V. Intertwined structure of the DNA-binding domain of intron endonuclease I-TevI with its substrate. EMBO J. (2001) 20, 3631-3637.
  • Gilson, M.A. and Belfort, M. Intron Homing. Encyclopedia of Genetics (2001) p. 1047-1051.
  • Cousineau, B., Lawrence, S., Smith, D. and Belfort, M. Retrotransposition of a bacterial group II intron. Nature (2000) 404,1018-1021. Correction. Nature (2001) 414, 84.
  • Mohr, G., Smith, D., Belfort, M., and Lambowitz, A.M. Rules for DNA target-site recognition by a lactococcal group II intron enable retargeting of the intron to specific DNA sequences. Genes Dev. (2000) 14, 559-573.
  • Lease, R. and Belfort, M. A trans-acting RNA as a control switch in E. coli: DsrA RNA modulates function by forming alternative structures. Proc. Natl. Acad. Sci. USA (2000) 97, 9919-9924.
  • Edgell, D., Shub, D. and Belfort, M. Barriers to intron promiscuity in bacteria. J. Bacteriol. (2000) 182, 5281-5289.
  • Lease, R. and Belfort, M. Riboregulation by DsrA RNA: trans-actions for global economy. Mol. Micro. (2000) 38, 667-672.
  • Wood, D.W., Derbyshire, V., Wu, W., Cartrain, M., Belfort, M., and Belfort, G. Optimized single-step affinity purification with a self-cleaving intein applied to human acidic fibroblast growth factor. Biotechnol. Prog. (2000) 16, 1055-1063.
  • Book (Editor)
  • Belfort, M., Derbyshire, V., Stoddard, B. and Wood, D. Homing Endonucleases and Inteins (2005) Springer Verlag.
  • Meeting Reviews
  • DiChiara, J. and Belfort, M. Interdisciplinary bacteria and phages. Genome Biology (2008) 9, 322.
  • Sweasy, J.B. and Belfort, M. The three Rs. Replication, recombination and repair in genome integrity, cancer and gene therapy. ASBMB Today (2010) July, 18-19.
  • Women in science publications
  • Belfort, M. The win-win potential for motherhood and science. Current Biology (2001) 11, R41-R42.
  • Belfort, M. Microbiological moms, their sisters and brothers: the give and take. ASM News (2003) 69, 553-557.