Digital Microscopy and Digital Imaging

The Biological Imaging Facility maintains excellent optics for a variety of imaging applications.

In addition to confocal imaging, the two methods for digital capture are provided within the Facility. First, the Zeiss LSM510 provides scanning lasers and a photomultiplier tube for capturing transmitted or DIC images on either inverted or upright microscopes. Second, a high-performance quantitative CCD camera and its associated computer provide high sensitivity monochrome imaging with 12-bit, low-noise output at 1360 x 1036 pixel resolution. Full resolution acquisition can occur at 5 fps. The camera is currently configured for controll through one of three software packages: Through itts own driver for adjustment and simple frame grabs, through SimplePCI from Compix for control requiring more features including control of the microscope, time-lapse and many image processing functions, and through ImageJ for those familiar with the NIH/Scion Image software.

Throughout the Department, video imaging systems based on Metamorph (Universal Imaging), Image (NIH/Scion) and Hamamatsu are used. Because of their integration with other experimental equipment, they are typically in individual research labs. High quality imaging of Calcium-sensitive fluorescence (Metafluor, Universal Imaging) is available on an Olympus inverted microscope.