Light microscopy

The Biological Imaging Facility supports individual laboratories by providing excellent, high-end optics and capability. The Facility has a Zeiss AxioPlan 2M upright microscope with 100W Halogen and 100W Mercury light sources and a Zeisss AxioVert 200M inverted microscope with 100W Halogen and 50W Mercury light sources. Fluorescence filter sets for most applications are available. Objectives include:

• 10X Fluar (NA 0.5, WD 2.0mm),
  
• 20X Plan ApoChromat (NA 0.75, WD 0.61),
  
• 40X AchroPlan (water, NA 0.8, WD 3.61mm),
  
• 40X C-ApoChromat (water with correction collar, NA 1.2, WD 0.29),
  
• 63X PlanApochromat (oil, NA 1.4, WD 0.18),
  
• 100X PlanNeofluar (oil, NA 1.3, WD 0.17).

Faculty in the Biology Department use a wide range of light microscopy techniques, including various methods of interference contrast, fluorescence, enhanced video microscopy and computer-assisted tracing.