Digital Microscopy and Photography

The Biological Imaging Facility maintains excellent optics for a variety of imaging applications. Although the Department maintains a complete photographic darkroom, researchers are increasingly making use of digital imaging and our facilities reflect that.

In addition to confocal imaging, the two methods for digital capture are provided within the Facility. First, the Zeiss LSM510 provides scanning lasers and a photomultiplier tube for capturing transmitted or DIC images on either inverted or upright microscopes. Second, a high-performance quantitative CCD camera (Retiga EX) and its associated computer provide high sensitivity monochrome imaging with 12-bit, low-noise output at 1360 x 1036 pixel resolution. Full resolution acquisition can occur at 5 fps. The camera is currently configured for controll through one of three software packages: Through itts own driver for adjustment and simple frame grabs, through SimplePCI from Compix for control requiring more features including control of the microscope, time-lapse and many image processing functions, and through ImageJ for those familiar with the NIH/Scion Image software.

Throughout the Department, video imaging systems based on Metamorph (Universal Imaging), Image (NIH/Scion) and Hamamatsu are used. Because of their integration with other experimental equipment, they are typically in individual research labs. High quality imaging of Calcium-sensitive fluorescence (Metafluor, Universal Imaging) is available on an Olympus inverted microscope.

The Photographic Laboratory contains film processors, copy stands and enlargers, our photographic suite now contains a flat bed scanner with transparency adapter (HP6100C/T), a film scanner (Nikon Coolscan III), a color inkjet printer (HP2200), and a film recorder/slide maker (Polaroid HR6000). Poster printing is provided by University Graphics.

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