Confocal Laser Scanning Microscopy

The Confocal facility contains a Zeiss LSM510/META scanning head, which can be positioned on either a Zeiss Axioplan2M upright microscope or an adjacent Zeiss Axiovert200M inverted microscope.

The LSM510 is capable of superb confocal imaging with a variety of filter sets to separate standard combinations of flourescent dyes. In addition, the META channel, which uses the entire spectrum of emitted light, allows discrimination between dyes that could not previously be separated. Laser lines at wavelengths of 458, 488, 514, 543 and 633 nm are available. A wide variety of filters and cubes are available.

An off-line workstation allows images to be viewed, manipulated and printed while the microscopes are in use.

For information about capabilites and use of this facility, contact Jeff Travis or Colin Izzard at the Department of Biological Sciences.