Applications - Biotechnology


Owen, T.S., Ngoje, G., Lageman, T., Belfort, M. and Callahan, B. FRET-based assay of hedgehog protein identifies cholesterolysis inhibitor. Analytic Biochemistry (2015) [Epub ahead of print].

Callahan, B., Stanger, M. and Belfort, M. A redox trap to augment the intein toolbox. Biotech. and Bioeng.(2013) 110, 1565-1573.


Callahan, B.P., Topilina, N., Stanger, M.J, Van Roey, P and Belfort, M. Catalytically competent intein caught in a redox trap: Structure, function, evolution. Nature Struc. Mol. Biol. (2011) 18, 630-633.

Callahan, B.P., Stanger, M.J. and Belfort, M. Protease activation of green fluorescent protein. Chembiochem. (2010) 11, 2259-2263.  

Zhang, L., Zheng, Y., Callahan, B., Belfort, M. and Liu, Y. Cisplatin inhibits protein splicing suggesting inteins as therapeutic targets in mycobacteria. J. Biol. Chem. (2010) 286, 1277-1282. Highlighted in Nature Chemical Biology.  


Silva, G.H., Belfort, M., Wende, W. and Pingoud, A. From Monomeric to Homodimeric Endonucleases and Back: Engineering Novel Specificity of LAGLIDADG Enzymes. J. Mol. Biol. (2006) 361, 744-754.  

Miao, J., Wu, W., Spielmann, T., Belfort, M., Derbyshire, V., and Belfort, G. Single-step affinity purification of toxic and non-toxic proteins on a fluidics platform. Lab on a Chip (2005) 5, 248-253.  


Novikova O, Smith D, Hahn I, Beauregard A, Belfort M. Interaction between conjugative and retrotransposable elements in horizontal gene transfer. PLoS Genet. (2014). 10 (12).

Jaeger J, Belfort M.  Hand-holding for retrohoming in a molecular diversity dance.  Structure. 2013 Feb 5;21(2):195-6.  


Belfort, M., Curcio, M.J., and Lue, N.F. Telomerase and retrotransposons: Reverse transcriptases that shaped genomes. Proc. Natl. Acad. Sci. USA (2011) 108, 20304-20310.  

Beauregard, A., Curcio, M.J. and Belfort, M. The take and give between retrotransposable elements and their hosts. Annu. Rev. Genetics. (2008) 42, 587-617.  

Curcio, M.J. and Belfort, M. The beginning of the end: Links between ancient retroelements and modern telomerases. Proc. Natl. Acad. Sci. USA (2007) 104, 9107-9108.


DiChiara, J.M., Contreras-Martinez, L.M., Livny, J., Smith, D., McDonough, K.A. and Belfort, M. Multiple small RNAs identified in Mycobacterium bovis BCG are also expressed in M. tuberculosis and M. smegmatis. Nucleic Acids Res. (2010) 38, 4067-4078.  

Lease, R.A., Smith, D., McDonough, K., and Belfort, M. The small non-coding DsrA RNA is an acid resistance regulator in Escherichia coli. J. Bacteriol. (2004) 186, 6179-6185.  



Robbins, J.B., Smith, D and Belfort, M. Redox-responsive zinc-finger is fidelity switch in homing endonuclease, promoting intron promiscuity under oxidative stress. Current Biol. (2011) 21, 243-248. 

Coros, C.J., Piazza, C. L., Chalamcharla, V.R., Smith, D. and Belfort, M. Global regulators orchestrate group II intron retromobility. Mol. Cell (2009) 34, 250-256. (Faculty of 1000, FFa8)