Publications
 

Publications

Applications - Biotechnology

Redox

Owen, T.S., Ngoje, G., Lageman, T., Belfort, M. and Callahan, B. FRET-based assay of hedgehog protein identifies cholesterolysis inhibitor. Analytic Biochemistry (2015) [Epub ahead of print].

Callahan, B., Stanger, M. and Belfort, M. A redox trap to augment the intein toolbox. Biotech. and Bioeng.(2013) 110, 1565-1573.

redox2

Callahan, B.P., Topilina, N., Stanger, M.J, Van Roey, P and Belfort, M. Catalytically competent intein caught in a redox trap: Structure, function, evolution. Nature Struc. Mol. Biol. (2011) 18, 630-633.

Callahan, B.P., Stanger, M.J. and Belfort, M. Protease activation of green fluorescent protein. Chembiochem. (2010) 11, 2259-2263.  

Zhang, L., Zheng, Y., Callahan, B., Belfort, M. and Liu, Y. Cisplatin inhibits protein splicing suggesting inteins as therapeutic targets in mycobacteria. J. Biol. Chem. (2010) 286, 1277-1282. Highlighted in Nature Chemical Biology.  

Intein

Silva, G.H., Belfort, M., Wende, W. and Pingoud, A. From Monomeric to Homodimeric Endonucleases and Back: Engineering Novel Specificity of LAGLIDADG Enzymes. J. Mol. Biol. (2006) 361, 744-754.  

Miao, J., Wu, W., Spielmann, T., Belfort, M., Derbyshire, V., and Belfort, G. Single-step affinity purification of toxic and non-toxic proteins on a fluidics platform. Lab on a Chip (2005) 5, 248-253.  



Retroelements

Novikova O, Smith D, Hahn I, Beauregard A, Belfort M. Interaction between conjugative and retrotransposable elements in horizontal gene transfer. PLoS Genet. (2014). 10 (12).

Jaeger J, Belfort M.  Hand-holding for retrohoming in a molecular diversity dance.  Structure. 2013 Feb 5;21(2):195-6.  

Belfort

Belfort, M., Curcio, M.J., and Lue, N.F. Telomerase and retrotransposons: Reverse transcriptases that shaped genomes. Proc. Natl. Acad. Sci. USA (2011) 108, 20304-20310.  

Beauregard, A., Curcio, M.J. and Belfort, M. The take and give between retrotransposable elements and their hosts. Annu. Rev. Genetics. (2008) 42, 587-617.  

Curcio, M.J. and Belfort, M. The beginning of the end: Links between ancient retroelements and modern telomerases. Proc. Natl. Acad. Sci. USA (2007) 104, 9107-9108.


sRNA’s

DiChiara, J.M., Contreras-Martinez, L.M., Livny, J., Smith, D., McDonough, K.A. and Belfort, M. Multiple small RNAs identified in Mycobacterium bovis BCG are also expressed in M. tuberculosis and M. smegmatis. Nucleic Acids Res. (2010) 38, 4067-4078.  

Lease, R.A., Smith, D., McDonough, K., and Belfort, M. The small non-coding DsrA RNA is an acid resistance regulator in Escherichia coli. J. Bacteriol. (2004) 186, 6179-6185.  


Stress

Par

Robbins, J.B., Smith, D and Belfort, M. Redox-responsive zinc-finger is fidelity switch in homing endonuclease, promoting intron promiscuity under oxidative stress. Current Biol. (2011) 21, 243-248. 

Coros, C.J., Piazza, C. L., Chalamcharla, V.R., Smith, D. and Belfort, M. Global regulators orchestrate group II intron retromobility. Mol. Cell (2009) 34, 250-256. (Faculty of 1000, FFa8)